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PromoCell endothelial cell growth media mv
RBC lysate (RBCL) or Hz from iRBC lysate (L-Hz; 18.88µg/cm 2 ) were added to a monolayer of primary human brain microvascular <t>endothelial</t> cells (HBMECs, A ), primary human cardiac microvascular endothelial cells (HCMECs, B ), or immortalized human pulmonary microvascular endothelial cells (HPMECs, C ). Barrier integrity of HBMEC monolayers was measured by xCELLigence as impedance every 15min over 24h. Bars represent barrier integrity at 24h. Results represent the average from 2 independent experiments performed in duplicate with standard deviations. Statistical significance was determined by unpaired t test where * = P < 0.05 and **** = P < 0.0001.
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R&D Systems endothelial cell growth media
Effect of extrusion process on μRB bioink and cell alignment. (A) Schematic of MSCs encapsulated in μRB bioink with HUVECs seeded on top of the printed scaffolds. (B) Live cell staining of MSC alignment on individual μRBs (Scale bar = 100 μm). (C) Distribution of MSC cell length (n = 250 per group); p-values were determined by one-way analysis of variance (ANOVA) with Tukey's multiple comparisons test; ∗∗∗∗p < 0.0001. (D) Cell orientation relative to μRB orientation, where 0° is parallel to the axis of the μRB (n = 250 per group). (E) Confocal images of F-Actin staining for cell morphology and VE-cadherin staining for <t>endothelial</t> cell junctions. Color survey visualization of directional analysis conducted using OrientationJ (Scale bar = 200 μm). (F, G) Quantification of F-actin and VE-Cadherin alignment. For alignment quantification (n = 10 per group) data reported as mean ± S.D., statistical analysis by Watson–Wheeler test, ∗p ≤ 0.05.
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R&D Systems endothelial cell growth supplement
Effect of extrusion process on μRB bioink and cell alignment. (A) Schematic of MSCs encapsulated in μRB bioink with HUVECs seeded on top of the printed scaffolds. (B) Live cell staining of MSC alignment on individual μRBs (Scale bar = 100 μm). (C) Distribution of MSC cell length (n = 250 per group); p-values were determined by one-way analysis of variance (ANOVA) with Tukey's multiple comparisons test; ∗∗∗∗p < 0.0001. (D) Cell orientation relative to μRB orientation, where 0° is parallel to the axis of the μRB (n = 250 per group). (E) Confocal images of F-Actin staining for cell morphology and VE-cadherin staining for <t>endothelial</t> cell junctions. Color survey visualization of directional analysis conducted using OrientationJ (Scale bar = 200 μm). (F, G) Quantification of F-actin and VE-Cadherin alignment. For alignment quantification (n = 10 per group) data reported as mean ± S.D., statistical analysis by Watson–Wheeler test, ∗p ≤ 0.05.
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R&D Systems endothelial growth factor vegf
Effect of extrusion process on μRB bioink and cell alignment. (A) Schematic of MSCs encapsulated in μRB bioink with HUVECs seeded on top of the printed scaffolds. (B) Live cell staining of MSC alignment on individual μRBs (Scale bar = 100 μm). (C) Distribution of MSC cell length (n = 250 per group); p-values were determined by one-way analysis of variance (ANOVA) with Tukey's multiple comparisons test; ∗∗∗∗p < 0.0001. (D) Cell orientation relative to μRB orientation, where 0° is parallel to the axis of the μRB (n = 250 per group). (E) Confocal images of F-Actin staining for cell morphology and VE-cadherin staining for <t>endothelial</t> cell junctions. Color survey visualization of directional analysis conducted using OrientationJ (Scale bar = 200 μm). (F, G) Quantification of F-actin and VE-Cadherin alignment. For alignment quantification (n = 10 per group) data reported as mean ± S.D., statistical analysis by Watson–Wheeler test, ∗p ≤ 0.05.
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R&D Systems endothelial cell growth base media
Effect of extrusion process on μRB bioink and cell alignment. (A) Schematic of MSCs encapsulated in μRB bioink with HUVECs seeded on top of the printed scaffolds. (B) Live cell staining of MSC alignment on individual μRBs (Scale bar = 100 μm). (C) Distribution of MSC cell length (n = 250 per group); p-values were determined by one-way analysis of variance (ANOVA) with Tukey's multiple comparisons test; ∗∗∗∗p < 0.0001. (D) Cell orientation relative to μRB orientation, where 0° is parallel to the axis of the μRB (n = 250 per group). (E) Confocal images of F-Actin staining for cell morphology and VE-cadherin staining for <t>endothelial</t> cell junctions. Color survey visualization of directional analysis conducted using OrientationJ (Scale bar = 200 μm). (F, G) Quantification of F-actin and VE-Cadherin alignment. For alignment quantification (n = 10 per group) data reported as mean ± S.D., statistical analysis by Watson–Wheeler test, ∗p ≤ 0.05.
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RBC lysate (RBCL) or Hz from iRBC lysate (L-Hz; 18.88µg/cm 2 ) were added to a monolayer of primary human brain microvascular endothelial cells (HBMECs, A ), primary human cardiac microvascular endothelial cells (HCMECs, B ), or immortalized human pulmonary microvascular endothelial cells (HPMECs, C ). Barrier integrity of HBMEC monolayers was measured by xCELLigence as impedance every 15min over 24h. Bars represent barrier integrity at 24h. Results represent the average from 2 independent experiments performed in duplicate with standard deviations. Statistical significance was determined by unpaired t test where * = P < 0.05 and **** = P < 0.0001.

Journal: bioRxiv

Article Title: Plasmodium falciparum hemozoin-associated biomolecules induce brain endothelial cell barrier disruption in an in vitro model of cerebral malaria

doi: 10.64898/2026.03.12.711413

Figure Lengend Snippet: RBC lysate (RBCL) or Hz from iRBC lysate (L-Hz; 18.88µg/cm 2 ) were added to a monolayer of primary human brain microvascular endothelial cells (HBMECs, A ), primary human cardiac microvascular endothelial cells (HCMECs, B ), or immortalized human pulmonary microvascular endothelial cells (HPMECs, C ). Barrier integrity of HBMEC monolayers was measured by xCELLigence as impedance every 15min over 24h. Bars represent barrier integrity at 24h. Results represent the average from 2 independent experiments performed in duplicate with standard deviations. Statistical significance was determined by unpaired t test where * = P < 0.05 and **** = P < 0.0001.

Article Snippet: HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90μg/mL heparin (PromoCell C-30120), 10ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1μg/mL hydrocortisone (PromoCell C-31061).

Techniques:

Effect of extrusion process on μRB bioink and cell alignment. (A) Schematic of MSCs encapsulated in μRB bioink with HUVECs seeded on top of the printed scaffolds. (B) Live cell staining of MSC alignment on individual μRBs (Scale bar = 100 μm). (C) Distribution of MSC cell length (n = 250 per group); p-values were determined by one-way analysis of variance (ANOVA) with Tukey's multiple comparisons test; ∗∗∗∗p < 0.0001. (D) Cell orientation relative to μRB orientation, where 0° is parallel to the axis of the μRB (n = 250 per group). (E) Confocal images of F-Actin staining for cell morphology and VE-cadherin staining for endothelial cell junctions. Color survey visualization of directional analysis conducted using OrientationJ (Scale bar = 200 μm). (F, G) Quantification of F-actin and VE-Cadherin alignment. For alignment quantification (n = 10 per group) data reported as mean ± S.D., statistical analysis by Watson–Wheeler test, ∗p ≤ 0.05.

Journal: Bioactive Materials

Article Title: Ribbon-shaped microgels as bioinks for 3D bioprinting of anisotropic tissue structures

doi: 10.1016/j.bioactmat.2025.12.040

Figure Lengend Snippet: Effect of extrusion process on μRB bioink and cell alignment. (A) Schematic of MSCs encapsulated in μRB bioink with HUVECs seeded on top of the printed scaffolds. (B) Live cell staining of MSC alignment on individual μRBs (Scale bar = 100 μm). (C) Distribution of MSC cell length (n = 250 per group); p-values were determined by one-way analysis of variance (ANOVA) with Tukey's multiple comparisons test; ∗∗∗∗p < 0.0001. (D) Cell orientation relative to μRB orientation, where 0° is parallel to the axis of the μRB (n = 250 per group). (E) Confocal images of F-Actin staining for cell morphology and VE-cadherin staining for endothelial cell junctions. Color survey visualization of directional analysis conducted using OrientationJ (Scale bar = 200 μm). (F, G) Quantification of F-actin and VE-Cadherin alignment. For alignment quantification (n = 10 per group) data reported as mean ± S.D., statistical analysis by Watson–Wheeler test, ∗p ≤ 0.05.

Article Snippet: Human umbilical vein endothelial cells (HUVECs, Lonza) were cultured in Endothelial Cell Growth Media (R&D Systems).

Techniques: Staining